PCR-RFLP analysis ofGiardia intestinalisusing aGiardia-specific gene, GLORF-C4
نویسندگان
چکیده
منابع مشابه
TaqI RFLP polymorphism of the ovine complement component C4 gene.
Protocol: Reactions were performed as described (2) using 1 ng of DNA and 100 pmoles of each primer. DNA was amplified for 20 cycles using a Perkin Elmer Cetus DNA Thermal Cycler: denaturation, 94°C, 1 min; annealing, 60°C, 1 min; and extension, 70°C, 1.5 min. Ten /il of the reaction was analyzed directly on a 2.5 % NuSieve GTG agarose gel run in Tns-borate buffer. The amplified DNA fragments v...
متن کاملGenotypic Analysis of Staphylococcus aureus Coagulase Gene using PCR-RFLP analysis
ABSTRACT Background and Objectives: Determining the genetic relationship between S. aureus isolates is important for epidemiological surveillance and control of infections caused by this bacterium. The present study was conducted to determine polymorphisms of coagulase gene (coa) among S. aureus isolates from pastry and cheese samples usi...
متن کاملIdentification of Avian Salmonella Isolates by PCR-RFLP Analysis of a fliC Gene Fragment
The genus of Salmonella is very polymorphic and comprised of a number of genetically closely related serotypes. It is one of the emerging pathogen in food-borne disease which is often found in contaminated chicken eggs. Salmonella enterica is considered one of the major pathogens in public health worldwide. A total of 31 Salmonella isolates identified by specific antisera, which included Salmon...
متن کاملکاربرد تکنیک های pcr-rflp وspecies-specific pcr برای شناسایی گونه های جایگزین درغذاهای دریایی
جایگزینی گونه های ماهی و غذاهای دریایی، به دلیل افزایش تجارت بین المللی، سرانه ی مصرف غذاهای دریایی و تولید غذاهای فرآوری شده، افزایش یافته و به یک نگرانی مهم در بازارهای داخلی و بین المللی تبدیل شده است. برای جلوگیری از جایگزینی غیرقانونی گونه ها، تعدادی از روشهای مبتنی بر dna توسعه یافته است که گونه های ماهی و غذاهای دریایی را در محصولات تجاری شناسایی می کند. این تکنیک ها شامل: pcr مرکب، pcr-...
Genotyping of Cryptosporidium spp. in clinical samples: PCR-RFLP analysis of the TRAP-C2 gene
AIM The aim of the present study was to determine the species and genotypes of Cryptosporidium spp. among children with diarrhea by PCR-RFLP using the TRAP-C2 gene. BACKGROUND Cryptosporidium is a globally distributed protozoan parasite and one of the most common causes of infection and diarrhea in humans. PATIENTS AND METHODS Four hundred and sixty nine stool samples were collected from ch...
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ژورنال
عنوان ژورنال: Parasite
سال: 2002
ISSN: 1252-607X,1776-1042
DOI: 10.1051/parasite/200209165